【文献标题】Prostaglandin Metabolome Profiles in Zebrafish (Danio rerio) Exposed to Acetochlor and Butachlor 【作者】Shenggan Wu , Xinzong Zhou , Weiwei Qin et.al 【作者单位】浙江农业科学院（Zhejiang Academy of Agricultural Sciences） 【文献中引用产品】 斑马鱼前列腺素（PGs）ELISA试剂盒 斑马鱼前列腺素E2（PGE2）ELISA试剂盒 斑马鱼前列腺素D2（PGD2）ELISA试剂盒 斑马鱼13,14二氢15-酮前列腺素F2α(PGFM)ELISA试剂盒 斑马鱼活性氧（ROS）ELISA试剂盒 【关键词】prostaglandins; metabolites; isoprostanes; Danio rerio; UPLC–ESI–MS/MS-metabolomics 【DOI】doi.org/10.3390/ijms24043488 【影响因子(IF)】5.59 【出版期刊】《International Journal of Molecular Sciences》 【产品原文引用】 The samples, mixed with eight females or males per repeat, were crushed and homogenized in the Phosphate Buffered Saline (PBS) solution (0.01 M, pH 7.4). Three repeat samples were used for each treatment. The homogenate was centrifuged at 5000 rpm/min for 10 min and the supernatant was separated. The collected supernatant was then stored at 80 C. The levels of the total PGs, PGE2, PGD2, 11β-13,14-dihydro-15-keto PGF2α (PGFM), 5-iPF2a-VI, and ROS were detected by commercial ELISA kits (Shanghai Hengyuan Biological Technology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions. These kits employed HRP-conjugate mouse antibodies to quantify PGs and ROS levels in samples. Statistical differences were subjected to one-way analysis of variance (ANOVA), followed by Tukey’s multiple comparison test. The statistical significance level was set at p < 0.05.
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