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恒远产品文献:人HGF,TGF-β ELISA试剂盒引用文献

  • 发布日期:2023/11/6      浏览次数:361
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    【文献标题】Hepatic Stellate Cells Secreted Hepatocyte Growth Factor Contributes to the Chemoresistance of Hepatocellular Carcinoma

    【作者】Guofeng Yu, Yingying Jing, Xingrui Kou, et.al

    【作者单位】上海第二军医大学(Second Military Medical University, Shanghai)

    【文献中引用产品】

    人肝细胞生长因子(HGF)ELISA试剂盒

    人转化生长因子β(TGF-β)ELISA试剂盒

    【DOI】doi:10.1371/journal.pone.0073312

    【影响因子(IF)】3.23

    出版期刊】《PLOS ONE》

    【产品原文引用】

    Conditioned medium and ELISA

    Cultured media (CM) were collected from LX-2 cells cultured in 2D culture dishes and centrifuged at 1000g for 5 minutes to obtain the supernatant. LX-2 CM was incubated with HGF antibody (GTX10678, 0.5 μg/ml) for 24h at 4oC to obtain HGFdepleted LX-2 CM. The level of HGF and TGF-β in CM waere determined by using human HGF ELISA kit (HY10158E, Shanghai HengYuan Biological Technology Co., Ltd) and TGF- β ELISA kit (HY10108E, Shanghai HengYuan Biological Technology Co., Ltd) according to manufacturer’s instructions.


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