恒远推荐：游离脂肪酸定量检测试剂盒

恒远推荐：游离脂肪酸定量检测试剂盒

Free Fatty Acid Quantification Colorimetric/Fluorometric Kit


游离脂肪酸，简称：FFA，存在于人体内的脂质，大致可以分为胆固醇、中性脂肪（三酸甘油脂）、磷脂质、游离脂肪酸等4种。游离脂肪酸是中性脂肪分解成的物质。当肌肉活动所需能源--肝糖耗尽时，脂肪组织会分解中性脂肪成为游离脂肪酸来充当能源使用。所以，游离脂肪酸可说是进行持久活动所需的物质。


Kit Summary:
? Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
? Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
? Species reactivity- Mammalian
? Application- The kit has a detection limit 2 μM free fatty acid in variety samples.

Features & Benefits:
? Simple procedure; takes ~40 minutes
? Fast and convenient
? Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Kit components:
? Fatty Acid Assay Buffer
? Fatty Acid Probe in DMSO
? ACS Reagent
? Enzyme Mix
? Enhancer
? Palmitic Acid Standard (1nmol/μl)

Description:
Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 μM free fatty acid in variety samples.

USAGE: For Research Use Only! Not For Use in Humans.

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