细胞色素P450(cytochromeP450或CYP450,简称CYP450)代表着一个很大的可自身氧化的亚铁血红素蛋白家族，属于单氧酶的一类，因其在450纳米有特异吸收峰而得名。它参与内源性物质和包括药物、环境化合物在内的外源性物质的代谢。根据氨基酸序列的同源程度，其成员又依次分为家族、亚家族和酶个体三级。细胞色素P450酶系统可缩写为CYP，其中家族以阿拉伯数字表示，亚家族以大写英文字母表示，酶个体以阿拉伯数字表示，如CYP2D6、CYP2C19、CYP3A4等。
研究意义:
细胞中，细胞色素P450主要分布在内质网和线粒体内膜上，作为一种末端加氧酶，参与了生物体内的甾醇类激素合成等过程。近年来，对细胞色素P450的结构、功能特别是对其在药物代谢中的作用的研究有了较大的进展。研究表明细胞色素P450是药物代谢过程中的关键酶，而且对细胞因子和体温调节都有重要影响。
相关文献:
【文献标题】Enantioselective toxicity and oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway
【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al
【作者单位】中国农业科学院烟草研究所(Tobacco Research Institute of Chinese Academy of Agricultural Sciences)
【文献中引用产品】
蚯蚓8-羟基脱氧鸟苷(8-OHdG)ELISA试剂盒
蚯蚓细胞色素P450(CYP450)ELISA试剂盒
蚯蚓羟基自由基(OH)ELISA检测试剂盒
【关键词】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic
【DOI】doi.org/10.1016/j.scitotenv.2020.142630
【影响因子(IF)】8.6
【出版期刊】《Science of the Total Environment》
【产品原文引用】
The OH content, 8-OhdG content, and CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, and activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle and method of 8-OhdG and CYP450 are the same as those of OH.
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