技术资讯:蚯蚓8-OHdG,OH,等ELISA试剂盒引用文献

【文献标题】Enantioselective toxicity and oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway
【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al
【作者单位】中国农业科学院烟草研究所（Tobacco Research Institute of Chinese Academy of Agricultural Sciences）
【文献中引用产品】
蚯蚓8-羟基脱氧鸟苷（8-OHdG）ELISA试剂盒
蚯蚓细胞色素P450(CYP450)ELISA试剂盒
蚯蚓羟基自由基(OH)ELISA检测试剂盒
【关键词】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic
【DOI】doi.org/10.1016/j.scitotenv.2020.142630
【影响因子(IF)】8.6
【出版期刊】《Science of the Total Environment》
【产品原文引用】
The OH content, 8-OhdG content, and CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, and activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle and method of 8-OhdG and CYP450 are the same as those of OH,


 
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